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Сloning and expression of catalytic subunit of mliii, the ribosome-inactivating protein from viscum albumстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Pevzner IB, Agapov II, Pfueller U., Pfueller K., Maluchenko NV, Moisenovich MM, Tonevitsky AG, Kirpichnikov MP
- Журнал: Biochemistry (Moscow)
- Том: 70
- Номер: 3
- Год издания: 2005
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 306
- Последняя страница: 315
- DOI: 10.1023/B:BIRY.0000033737.79960.a7
- Аннотация: Aqueous extracts of mistletoe (Viscum album L.) contain toxic proteins (lectins) MLI (viscumin), MLII, and MLIII. We previously cloned the gene encoding MLIII precursor. In the present study, a gene fragment encoding the carbohydrate-binding subunit of mistletoe toxic lectin MLIII was cloned and expressed in Escherichia coli cells. The structure and immunochemical properties of recombinant MLIII B-subunit were investigated using a panel of monoclonal antibodies against ML-toxins. Sugar-binding activity of recombinant MLIII B-subunit was determined by ELISA. Amino acid sequence analysis of the cloned MLIII compared with known mistletoe toxins and other ribosome-inactivating type II proteins (ricin, abrin a, and nigrin b B-subunits) revealed essential features of the recombinant MLIIIB primary structure that could determine sugar specificity of the lectin as well as immunomodulating and anti-tumor properties of mistletoe extracts
- Добавил в систему: Малюченко Наталия Валериевна