|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Profiling of Mycoplasma gallisepticum Ribosomesстатья
Статья опубликована в журнале из списка Web of Science и/или Scopus
- Авторы: Fisunov GY, Evsyutina DV, Arzamasov AA, Butenko IO, Govorun VM
- Журнал: Acta Naturae (англоязычная версия)
- Год издания: 2016
- Издательство: Park Media Ltd
- Местоположение издательства: Moscow, Russia (Federation)
- Аннотация: The development of high-throughput technologies is increasingly resulting in identification of numerous cases of low correlation between mRNA and the protein level in cells. These controversial observations were made on various bacteria, such as E. coli, Desulfovibrio vulgaris, and Lactococcus lactis. Thus, it is important to develop technologies, including high-throughput techniques, aimed at studying gene expression regulation at the level of translation. In the current study, we performed proteomic profiling of M. gallisepticum ribosomes and identified high abundant noncanonical proteins. We found that binding of mRNAs to ribosomes is mainly determined by two parameters: (1) abundance of mRNA itself and (2) complimentary interactions between the 3’ end of 16S rRNA and the ribosome binding site in the 5’-untranslated region of mRNA
- Добавил в систему: Евсютина Дарья Викторовна