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Cell-type-restricted anti-cytokine therapy: TNF inhibition from one pathogenic sourceстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 мая 2016 г.
- Авторы: Efimov GA, Kruglov AA, Khlopchatnikova ZV, Rozov FN, Mokhonov VV, Rose-John S., Scheller J., Gordon S., Stacey M., Drutskaya MS, Tillib SV, Nedospasov SA
- Журнал: Proceedings of the National Academy of Sciences of the United States of America
- Том: 113
- Номер: 11
- Год издания: 2016
- Издательство: National Academy of Sciences
- Местоположение издательства: United States
- Первая страница: 3006
- Последняя страница: 3011
- DOI: 10.1073/pnas.1520175113
- Аннотация: Overexpression of TNF contributes to pathogenesis of multiple autoimmune diseases, accounting for a remarkable success of antiTNF therapy. TNF is produced by a variety of cell types, and it can play either a beneficial or a deleterious role. In particular, in autoimmunity pathogenic TNF may be derived from restricted cellular sources. In this study we evaluated the feasibility of cell-type–restricted TNF inhibition in vivo. To this end, we engineered MYSTI (Myeloid-Specific TNF Inhibitor)—a recombinant bispecific antibody that binds to the F4/80 surface molecule on myeloid cells and to human TNF (hTNF). In macrophage cultures derived from TNF humanized mice MYSTI could capture the secreted hTNF, limiting its bioavailability. Additionally, as evaluated in TNF humanized mice, MYSTI was superior to an otherwise analogous systemic TNF inhibitor in protecting mice from lethal LPS/D-Galactosamine–induced hepatotoxicity. Our results suggest a novel and more specific approach to inhibiting TNF in pathologies primarily driven by macrophage-derived TNF.
- Добавил в систему: Шилов Евгений Сергеевич