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Binding site of MraZ transcription factor in Mollicutesстатья
- Авторы: Fisunov GY, Evsyutina DV, Semashko TA, Arzamasov AA, Manuvera VA, Letarov AV, Govorun VM
- Журнал: Biochimie
- Год издания: 2016
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- DOI: 10.1016/j.biochi.2016.02.016
- Аннотация: Mollicutes (mycoplasmas) feature a significant loss of known regulators of gene expression. Here, we identified the recognition site of the MraZ-family regulator of Mycoplasma gallisepticum, which is conserved in many species of different clades within class Mollicutes. The MraZ binding site is AAAGTG[T/G], in the promoter of mraZ gene it forms a series of direct repeats with a structure (AAAGTG[T/G]N3)k, where k = 3 most frequently. MraZ binds to a single repeat as an octamer complex. MraZ can also bind a single binding site or a series of repeats with different spacer lengths (2-4 nt); thus, it may play a role in the regulation of multiple operons in Mollicutes. In M. gallisepticum, MraZ acts as a transcriptional activator. The overexpression of MraZ leads to moderate filamentation of cells and the formation of aggregates, likely as a result of incomplete cytokinesis.
- Добавил в систему: Евсютина Дарья Викторовна