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Nox4 and Duox1/2 Mediate Redox Activation of Mesenchymal Cell Migration by PDGFстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 июня 2016 г.
- Авторы: Tyurin-Kuzmin Pyotr A., Zhdanovskaya Nadezhda D., Sukhova Anna A., Sagaradze George D., Albert Eugene A., Ageeva Ludmila V., Sharonov George V., Vorotnikov Alexander V., Tkachuk Vsevolod A.
- Журнал: PLoS ONE
- Том: 11
- Номер: 4
- Год издания: 2016
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: e0154157
- DOI: 10.1371/journal.pone.0154157
- Аннотация: Platelet derived growth factor (PDGF) orchestrates wound healing and tissue regeneration by regulating recruitment of the precursor mesenchymal stromal cells (MSC) and fibroblasts. PDGF stimulates generation of hydrogen peroxide that is required for cell migration, but the sources and intracellular targets of H2O2 remain obscure. Here we demonstrate sustained live responses of H2O2 to PDGF and identify PKB/Akt, but not Erk1/2, as the target for redox regulation in cultured 3T3 fibroblasts and MSC. Apocynin, cell-permeable catalase and LY294002 inhibited PDGF-induced migration and mitotic activity of these cells indicating involvement of PI3-kinase pathway and H2O2. Real-time PCR revealed Nox4 and Duox1/2 as the potential sources of H2O2. Silencing of Duox1/2 in fibroblasts or Nox4 in MSC reduced PDGF-stimulated intracellular H2O2, PKB/Akt phosphorylation and migration, but had no such effect on Erk1/2. In contrast to PDGF, EGF failed to increase cytoplasmic H2O2, phosphorylation of PKB/Akt and migration of fibroblasts and MSC, confirming the critical impact of redox signaling. We conclude that PDGF-induced migration of mesenchymal cells requires Nox4 and Duox1/2 enzymes, which mediate redox-sensitive activation of PI3-kinase pathway and PKB/Akt.
- Добавил в систему: Тюрин-Кузьмин Петр Алексеевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Tyurin-Kuzmin_et_al._2016.PDF | 4,6 МБ | 27 апреля 2016 [Tyurinkuzmin] |