Hydrogen peroxide affects ion channels in lily pollen grain protoplastsстатьяИсследовательская статья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 2 октября 2018 г.
Аннотация:Ion homeostasis plays a central role in polarization and polar growth. In several cell types ion channels are known to be controlled by reactive oxygen species. One of the most important cells in plant life cycle is male gametophyte, which is growing under the tight control of both ion fluxes and ROS balance. The precise relationship between these two factors in pollen tubes has not been completely understood, and in pollen grains has never been studied to date. In the present study we used a simple model - protoplasts obtained from lily pollen grains at the early germination stage - to reveal the effect of H2O2 on cation fluxes crucial for pollen germination. Here we present the direct evidence for the two ROS-sensitive currents on the pollen grain plasma membrane: the hyperpolarization-activated calcium current which is strongly enhanced by H2O2 and the outward potassium current which is modestly enhanced by H2O2 . We used low concentrations of H2O2 which do not cause intracellular oxidative burst and do not damage cells as demonstrated by fluorescent staining