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Light scattering study of the antibody-poly(methacrylic acid) and antibody-poly(acrylic acid) conjugates in aqueous solutionsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Kazakov S.V., Muronetz V.I., Dainiak M.B., Izumrudov V.A., Galaev I.Y., Mattiasson B.
- Журнал: Macromolecular Bioscience
- Том: 1
- Номер: 4
- Год издания: 2001
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 157
- Последняя страница: 163
- DOI: 10.1002/1616-5195(20010601)1:4<157::AID-MABI157>3.0.CO;2-S
- Аннотация: The effect of the conformational state of the polymer coil on the properties of protein-polymer conjugates has been studied for the conjugates of antibody (monoclonal antibody from 6C5 clone against inactivated rabbit muscle glyceraldehyde-3-phosphate dehydrogenase; Ab) with poly(methacrylic acid) (PMAA) or poly- (acrylic acid) (PAA). The pH-dependencies of molecular properties and structural parameters of aqueous solutions (radius of gyration, intensity of scattered light, hydrodynamic diameter, and polydispersity index). of Ab, PMAA, and PAA and their conjugates, i.e., Ab-PMAA and Ab-PAA have been studied using static and dynamic light-scattering techniques. While free Ab aggregates in solution and precipitates at its isoelectric point, the covalent attachment of a charged polymer to Ab prevents-its association and shifts the precipitation point towards more acidic values (from pH 5.95 for Ab to pH similar to4.8 for Ab-PMAA). The predominant role of the conformational status of the polymer in the process of conjugate precipitation has been considered. Contrary to the precipitation of Ab-PMAA, the formation of stable colloidal particles was suggests for Ab-PAA at PH <4.8. In the conjugates, polymer chains surround the protein globule in an extremely compact manner while Ab significantly affects the polymer conformation. The essentially larger hydrodynamic radii of conjugates, when compared with their radii of gyration, confirm the strong interaction of conjugates with solvent molecules. [GRAPHICS] pH-Dependencies of the radii of gyration for the solutions of Ab-PMAA and AB-PAA conjugates. The dotted arrow indicates that calculation of the radius of gyration at pH >4 has not been fulfilled for the solution of Ab-PAA conjugates.
- Добавил в систему: izumrudov