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Analysis of novel hyperosmotic shock response suggests beads-in-liquid cytosol structureстатья Исследовательская статья
- Авторы: Alexandrov Alexander I., Grosfeld Erica V., Dergalev Alexander A., Kushnirov Vitaly V., Chuprov-Netochin Roman N., Tyurin-Kuzmin Pyotr A., Kireev Igor I., Ter-Avanesyan Michael D., Leonov Sergey V., and Michael O.Agaphonov
- Журнал: Biology Open
- Том: 8
- Год издания: 2019
- Издательство: The Company of Biologists Ltd.
- Местоположение издательства: United Kingdom
- DOI: 10.1242/bio.044529
- Аннотация: Proteins can aggregate in response to stresses, including hyperosmotic shock. Formation and disassembly of aggregates is a relatively slow process. We describe a novel instant response of the cell to hyperosmosis, during which chaperones and other proteins form numerous foci with properties uncharacteristic of classical aggregates. These foci appeared/disappeared seconds after shock onset/removal, in close correlation with cell volume changes. Genome-wide and targeted testing revealed chaperones, metabolic enzymes, P-body components and amyloidogenic proteins in the foci. Most of these proteins can form large assemblies and for some, the assembled state was pre-requisite for participation in foci. A genome-wide screen failed to identify genes whose absence prevented foci participation by Hsp70. Shapes of and interconnections between foci, revealed by super-resolution microscopy, indicated that the foci were compressed between other entities. Based on our findings, we suggest a new model of cytosol architecture as a collection of numerous gel-like regions suspended in a liquid network. This network is reduced in volume in response to hyperosmosis and forms small pockets between the gel-like regions.
- Добавил в систему: Киреев Игорь Игоревич