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[Production of hepatitis B virus core particles protein in plants, by using cowpea mosaic virus-based vector]статья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 февраля 2014 г.
- Авторы: Meshcheriakova I.u.A, El’darov M.A., Beales L., Skriabin K.G., Lomonossoff G.P.
- Журнал: Voprosy Virusologii
- Том: 53
- Номер: 3
- Год издания: 2008
- Издательство: Meditsina Publishers
- Местоположение издательства: Russian Federation
- Первая страница: 15
- Последняя страница: 20
- Аннотация: The core antigen of hepatitis B virus (HBcAg) has attracted considerable attention as a carrier for antigenic sequences for various diagnostic and vaccine applications. The hepatitis B core protein has been expressed in different expression systems. At present, for reasons of cost, scale, and safety, the plant-based expression systems are attracting increasing interest. The expression and assembly for the hepatitis B core protein were investigating in N. benthamiana plants using the new expression system based on deleted version of cowpea mosaic virus RNA-2. Analysis of HBcAg expression revealed that the core protein expressed in plants and could self-assemble into virus-like particles. Virus-like particles could be purified by differential and sucrose gradient centrifugation. This expression system has the advantage of biocontainment and can be used for the rapid production of HBcAg virus-like particles for immunological and vaccine applications.
- Добавил в систему: Эльдаров Михаил Анатольевич