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Structural modeling of tobacco mosaic virus and its coat protein according small angle X- ray scattering dataстатья Тезисы
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 декабря 2019 г.
- Авторы: Ksenofontov A., Petoukhov M., Fedorova N., Ksenofontova O., Dobrov E., Shtykova E.
- Журнал: FEBS open bio
- Том: 9
- Номер: Suppl. 1
- Год издания: 2019
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 260
- Последняя страница: 261
- DOI: 10.1002/2211-5463.12675
- Аннотация: Tobacco mosaic virus (TMV) has been extensively studied in the past using crystallographic and electron microscopy approaches. In the present work structuralcharacterization and modeling of TMV and viruslike particles (VLP) formed by the virus coat protein (CP) was performed using synchrotron smallangle Xray scattering (SAXS). Scattering profiles from the samples in solution were collected at the P12 BioSAXS beamline at EMBL Hamburg. As a structural template for thevirus modeling the EM structure (2OM3.PDB) containing a fragment with helical symmetry has been chosen. The reconstructed rodlike model reasonably fits theexperimental SAXS patterns. TMV CP selfassembles in solution forming VLP. Structural modeling of these VLPs was performed using a fragment of crystallographicmodel of the fourlayer aggregate (1EI7.PDB). The resulting VLP’s model was represented by hollow cylinder yielding a good fit to the experimental SAXS data. Thus,for the first time, low resolution structures of TMV and viruslike particles were determined in solution, i.e. under conditions close to natural. However, solution structureof the individual TMV coat protein is still undefined due to its higher tendency to aggregation. One of the ways to solve the problem is to use dissociation by surfactants.In the present work highly stable partially unfolded intermediates (at low ionic strength and 42°C) were investigated by SAXS and complementary spectral methods. Itwas found that these intermediates retain about half of the initial content of the secondary and tertiary structure. Further dissociation was obtained by incubation at 42°Cwith 3.5 mM sodium dodecyl sulfate (SDS) without changes in tertiary structure, while incubation with 17.3 mM SDS resulted in a complete loss of the tertiary structureleading to a formation of the wellknown “necklace and bead” organization of CP in solution. The work was supported by RFBR grants 180400525 and 170000487.
- Добавил в систему: Ксенофонтов Александр Леонидович