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Site-specific N-glycosylation of windmill palm tree peroxidase using novel tools for analysis of plant glycopeptide mass spectrometry dataстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 июля 2016 г.
- Авторы: Baker M.R., Tabb D.L., Ching T., Zimmerman L.J., Sakharov I.Yu, Li Q.X.
- Журнал: Journal of Proteome Research
- Том: 15
- Номер: 6
- Год издания: 2016
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 2026
- Последняя страница: 2038
- DOI: 10.1021/acs.jproteome.6b00205
- Аннотация: Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree (Trachycarpus fortunei) peroxidase are reported. The workflow developed in this study includes novel tools, written in R, to aid plant glycan identification, pGlycoFilter, for annotation of glycopeptide fragmentation spectra, gPSMvalidator, and for relative quantitation of glycoforms, glycoRQ. Mass spectrometry analysis provided a detailed glycosylation profile at the 13 sites of N-linked glycosylation on windmill palm tree peroxidase. Glycan microheterogeneity was observed at each site. Site Asn211 was the most heterogeneous and contained 30 different glycans. Relative quantitation revealed 90% of each glycosylation site was occupied by three or fewer glycans, and two of the 13 sites were partially unoccupied. Although complex and hybrid glycans were identified, the majority of glycans were paucimannosidic, characteristic of plant vacuolar glycoproteins. Further studies pertaining to the glycan structure-activity relationships in plant peroxidases can benefit from the work outlined here.
- Добавил в систему: Сахаров Иван Юрьевич