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Endogenous peptides from the Physcomitrella patens mossстатья
- Авторы: Skripnikov A., Barannik A.P., Ziganshin R.H., Kharybin O., Polyakov N.B., Nikolaev E.N., Govorun V.M.
- Сборник: Abstracts of the XVI Congress of the Federation of European Societies of Plant Biology
- Серия: Physiologia Plantarum
- Том: 3
- Год издания: 2008
- Место издания: Blackwell Publishing Inc United Kingdom
- Первая страница: P13
- Аннотация: Peptides are major signaling molecules in animals and yeasts. In plants, only a few peptides are known that control physiology, growth and development. Full sequencing of genome of the moss Physcomitrella patens (Hedw.) B.S.G. together with recent advances in peptide mass-spectrometry and bioinformatics opened new possibilities for conducting genome-wide search of peptides that perform signaling role in plants. In attempt to clarify the role of peptides in moss physiology we started our studies on isolation, identifi cation and further elucidation of physico-chemical and biological properties of peptides from protoplasts, protonemata and gametophores of Physcomitrella patens. We have elaborated the strategy of extraction and fractionation of peptide-containing material from moss protoplasts and tissues. Total mass-spectral analysis by MALDI-TOF-TOF of HPLC fractions revealed over 400 individual components with molecular masses below 10 kD. Individual fractions were analyzed by LC-MS-MS FTI CR mass-spectrometry. A number of moss endogenous peptides has been sequenced mainly derived from chloroplast proteins.
- Добавил в систему: Скрипников Александр Юрьевич