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[Insertion of (dA-dT)n sequences into the regulatory region of the pho5 gene inhibits its expression]статья
Статья опубликована в журнале из списка RSCI Web of Science
Статья опубликована в журнале из перечня ВАКСтатья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Sidorova I.u.M, Kistanova E.N., Chernov B.K., El’darov M.A., Skriabin K.G., Nikiforov V.G., Mirkin S.M.
- Журнал: Молекулярная биология
- Том: 24
- Номер: 1
- Год издания: 1990
- Первая страница: 163
- Последняя страница: 172
- Аннотация: We have studied the effect of some regular sequences namely (dA-dT)n, (dA)n and (dG-dC)n on the Saccharomyces cerevisiae PHO5 gene expression. These sequences were inserted into the ClaI site, located between two phosphate-responsible upstream activating sequences (UAS’s). A modified PHO5 gene cloned in vector plasmids was used to transform the pho3, pho5 yeast strain and the level of acid phosphatase expression in various conditions was measured. We show that the insertion of (dA-dT)n blocks, but not (dA)n or (dG-dC)n, leads to the dramatic decrease of PHO5 gene expression in a derepressed state. (dA-dT)n inserts also inhibit the expression of PHO5 gene which was put under the control of heterologous UASgal.
- Добавил в систему: Эльдаров Михаил Анатольевич