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Explicit measurement of the endotoxin adsorption efficiency detects non-Langmuir behavior at low concentrationsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 марта 2020 г.
- Авторы: Kholina Ekaterina G., Nesterenko Alexey M., Galochkina Tatiana V., Gvozdev Danil A., Polyakova Irina V., Kovalenko Ilya B., Strakhovskaya Marina G., Pisarev Oleg A.
- Журнал: Analytical Biochemistry
- Том: 587
- Год издания: 2019
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 113445
- DOI: 10.1016/j.ab.2019.113445
- Аннотация: Lipopolysaccharides (LPS) are the Gram-negative bacteria cell wall components capable to induce the system inflammatory response even at picomolar concentrations. LPS detection at these concentrations is necessary to develop new sorbents for the efficient purification of the biological fluids. LAL-test widely used for LPS concentration estimation is based on the LPS biological activity measurement and thus may depend on the LPS concentration in a non-linear way. Here we propose a new explicit method for the LPS concentration measurement based on fluorescently labeled LPS and direct photon counting and develop the new protocol for LPS adsorption efficiency measurement. Following the suggested protocol in the experiments on novel sorbents, we demonstrate that LPS adsorption at small biologically relevant concentrations is non-Langmuir.
- Добавил в систему: Нестеренко Алексей Михайлович