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Differential Diagnostics of Active Progressing Multiple Sclerosis Using a Fluorescent Biomarker with Resonance Energy Transferстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 ноября 2019 г.
- Авторы: Lomakin Ya A., Kudryaeva A.A., Kostin N.N., Terekhov S.S., Kaminskaya A.N., Zakharova M.N., Ivanova M.V., Simaniv T.O., Gabibov A.G., Belogurov A.A.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 167
- Номер: 3
- Год издания: 2019
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 329
- Последняя страница: 334
- DOI: 10.1007/s10517-019-04520-1
- Аннотация: Previous data showed that myelin-reactive autoantibodies found in patients with multiple sclerosis and mice with experimental autoimmune encephalomyelitis recognize and hydrolyze various fragments of myelin basic protein (MBP). Moreover, antibody-mediated cleavage of the encephalithogenic fragment MBP81-103 flanked with two fluorescent proteins can serve as a new biomarker of multiple sclerosis. Here we describe creation of the next generation of this biomarker based on antibody-dependent degradation of a new chemically synthesized fluorescent substrate with resonance energy transfer that contains fluorophore Cy5 and quencher QXL680 separated by MBP81-99 protein (Cy5-MBP81-99-QXL680). This substrate is degraded during incubation with purified antibodies and B cells from patients with multiple sclerosis, but not healthy volunteers.
- Добавил в систему: Смирнова Инна Григорьевна