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Automated Analysis of Cell Cycle Phase-Specific DNA Damage Reveals Phase-Specific Differences in Cell Sensitivity to Etoposideстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 сентября 2017 г.
- Авторы: Luzhin AV, Velichko AK, Razin SV, Kantidze OL
- Журнал: Journal of Cellular Biochemistry
- Том: 117
- Номер: 10
- Год издания: 2016
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 2209
- Последняя страница: 2214
- DOI: 10.1002/jcb.25615
- Аннотация: The comet assay is one of the most widely used approaches for detecting DNA damage; generally, it provides information on the cell population-averaged level of DNA damage. Here, we present an automatic technique for easy measurement of standard comet characteristics and an annotation of the cell cycle phase of each comet. The approach includes the modified neutral comet assay and a pipeline for CellProfiler software designed to analyze DNA damage-related characteristics and annotate the cell cycle phase of each comet. Using this technique we have performed cell cycle phase-specific analysis of DNA damage induced by the topoisomerase II poison etoposide and have shown that the sensitivity of cells to this drug dramatically differed according to their cell cycle phase. It became evident from our results that the proposed protocol provides important additional information that often remains hidden in a standard comet analysis of an asynchronous cell population.
- Добавил в систему: Разин Сергей Владимирович