|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Acetylation of histones in neocortex and hippocampus of rats exposed to different modes of hypobaric hypoxia: Implications for brain hypoxic injury and toleranceстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 ноября 2019 г.
- Авторы: Samoilov M., Churilova A., Gluschenko T., Vetrovoy O., Dyuzhikova N., Rybnikova E.
- Журнал: Acta Histochemica
- Том: 118
- Номер: 2
- Год издания: 2016
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 80
- Последняя страница: 89
- DOI: 10.1016/j.acthis.2015.11.008
- Аннотация: Acetylation of nucleosome histones results in relaxation of DNA and its availability for the transcrip-tional regulators, and is generally associated with the enhancement of gene expression. Although it iswell known that activation of a variety of pro-adaptive genes represents a key event in the developmentof brain hypoxic/ischemic tolerance, the role of epigenetic mechanisms, in particular histone acetylation,in this process is still unexplored. The aim of the present study was to investigate changes in acetylationof histones in vulnerable brain neurons using original well-standardized model of hypobaric hypoxia andpreconditioning-induced tolerance of the brain. Using quantitative immunohistochemistry and Westernblot, effects of severe injurious hypobaric hypoxia (SH, 180 mm Hg, 3 h) and neuroprotective precondi-tioning mode (three episodes of 360 mm Hg for 2 h spaced at 24 h) on the levels of the acetylated proteinsand acetylated H3 Lys24 (H3K24ac) in the neocortex and hippocampus of rats were studied. SH causedglobal repression of the acetylation processes in the neocortex (layers II–III, V) and hippocampus (CA1,CA3) by 3–24 h, and this effect was prevented by the preconditioning. Moreover, hypoxic precondition-ing remarkably increased the acetylation of H3K24 in response to SH in the brain areas examined. Thepreconditioning hypoxia without subsequent SH also stimulated acetylation processes in the neocortexand hippocampus. The moderately enhanced expression of the acetylated proteins in the preconditionedrats was maintained for 24 h, whereas acetylation of H3K24 was intense but transient, peaked at 3 h. Thenovel data obtained in the present study indicate that large activation of the acetylation processes, inparticular acetylation of histones might be essential for the development of brain hypoxic tolerance.
- Добавил в систему: Ветровой Олег Васильевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|