|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Morrbid lncRNA is related to pre-mRNA processing in hepatocytesтезисы доклада Тезисы
Информация о цитировании статьи получена из
Web of Science,
Scopus
Дата последнего поиска статьи во внешних источниках: 23 января 2020 г.
- Авторы: Fefilova A., Sergeeva O., Nazarova M., Melnikov P., Kireev I., Zatsepin T.
- Сборник: FEBS Open Bio
- Серия: Suppl. 1
- Том: 9
- Тезисы
- Год издания: 2019
- Место издания: John Wiley & Sons Inc United States
- Первая страница: 39
- Последняя страница: 39
- DOI: 10.1002/2211-5463.12674
- Аннотация: Long noncoding RNAs (lncRNA) are RNA molecules longer than 200 nucleotides, which do not code for protein and represent the most abandon part of the transcribed genome. LncRNAs have multiple functions in various cellular processes, such as chromatin modeling, transcription, splicing, protein translation, etc. Some lncRNA have several transcripts, which may possess tissue specificity or have different functions depending on conditions. LncRNA Morrbid regulates the life span of neutrophils, eosinophils and classical monocytes through transcriptional regulation of its neighboring proapoptotic gene Bcl2l11 in mice (Kotzin et al, Nature 2016). In our work, we demonstrate that downregulation of Morrbid lncRNA in mouse hepatocytes results in stagnation of cell cycle in G2/M transition phase. Proteome analysis of Morrbid depleted cells uncovered significant downregulation of proteins involved in premRNA processing including hnRNP protein family and several proteins from U1 and U2 snRNP complexes. We demonstrated that Morrbid lncRNA interacts with some proteins involved in regulation of the premRNA 3’end processing using RNA pulldown assay. Thus, we found that Morrbid lncRNA may participate in the regulation of premRNA maturation and splicing in hepatocytes.
- Добавил в систему: Киреев Игорь Игоревич