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Enzymatic Synthesis of 2-Deoxyribose 1-Phosphate and Ribose 1-Phosphate and Subsequent Preparation of Nucleosidesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 декабря 2019 г.
- Авторы: Kulikova Irina V., Drenichev Mikhail S., Solyev Pavel N., Alexeev Cyril S., Mikhailov Sergey N.
- Журнал: European Journal of Organic Chemistry
- Том: 2019
- Номер: 41
- Год издания: 2019
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 6999
- Последняя страница: 7004
- DOI: 10.1002/ejoc.201901454
- Аннотация: α‐Ribose‐1‐phosphate (Rib‐p) and 2‐deoxy‐α‐ribose‐1‐phosphate (dRib‐p) are key intermediates in nucleoside metabolism and are important starting compounds for the enzymatic synthesis of various modified nucleosides. To date, chemical and enzymatic methods allowed the preparation of these compounds in rather low yields (11–37 %). This prevents their widespread use for the enzymatic synthesis of biologically active and practically important nucleosides. Here we propose to use 7‐methyl‐2′‐deoxyguanosine (7‐Me‐dGuo) and 7‐methylguanosine (7‐Me‐Guo) for the preparation of dRib‐p and Rib‐p. In this paper, we present the effective preparation of Rib‐p and dRib‐p starting from readily prepared 7‐methylguanosine derivatives via their irreversible enzymatic phosphorolysis in the presence of purine nucleoside phosphorylase. Rib‐p and dRib‐P are obtained in nearly quantitative yields (HPLC analysis) and 74–96 % yields after their isolation and purification, which is much higher than previously reported.
- Добавил в систему: Сольев Павел Николаевич