|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
DEPRESSION OF THE RADIOPROTECTIVE EFFECT OF ISOPROTERENOL ON MAMMALIAN-CELLS INVITRO AFTER DESENSITIZATION OF THE CAMP SYSTEMстатья Исследовательская статья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: CHIRKOV YY, MALATSIDZE MA, KAZAROV AR, SOBOLEV AS
- Журнал: International Journal of Radiation Biology
- Том: 48
- Номер: 5
- Год издания: 1985
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- Первая страница: 753
- Последняя страница: 760
- DOI: 10.1080/09553008514551851
- Аннотация: A short (5 min) incubation of cultured Chinese hamster fibroblasts with the specific β-agonist isoproterenol (1 μm) leads to an increase in the intracellular content of cAMP and a decrease in radiosensitivity of the cells. Prolonged (up to 1 h) incubation induces a desensitization of the cAMP system to isoproterenol and causes a decrease both in the cAMP-stimulating and radioprotective effect of isoproterenol. There were no detectable changes in the β-adrenoreceptor number or binding affinity of β-receptors to the radiolabelled β-antagonist dihydroalprenolol in desensitized cells; cAMP-phosphodiesterase activity was also the same as in intact cells. It is proposed that a 1 h incubation of the cells with isoproterenol induces the first step of desensitization, i.e. the functional ‘uncoupling’ of β-receptors from adenylate cyclase. Thus, the presence of β-receptors in cells is not enough for the realization of the radioprotective potency of isoproterenol; an intact, non-desensitized, state of the cAMP system is obligatory.
- Добавил в систему: Соболев Александр Сергеевич