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Development of real-time PCR assay for genotyping SNP rs41255693 in cattle SCD geneстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2021 г.
- Авторы: Kovalchuk Svetlana N., Arkhipova Anna L., Klimov Eugene A.
- Журнал: AIMS AGRICULTURE AND FOOD
- Том: 5
- Номер: 1
- Год издания: 2020
- Первая страница: 14
- Последняя страница: 19
- DOI: 10.3934/agrfood.2020.1.14
- Аннотация: Increased content of monounsaturated fatty acids with low melting points in cattle milk and meat known to strongly affect their taste and nutritional value. Steroyl-CoA-desaturase (SCD) is a key enzyme in the biosynthesis of monounsaturated fatty acids. The gene encoding SCD in cattle is located on chromosome 26 and consists of 6 exons and 5 introns and is expressed in the cells of cow udder, adipose, muscles and other tissues. To date, several SNPs in cattle SCD gene have been found. For SNP rs41255693 (g.10329T > C), a correlation was shown between the content of monounsaturated fatty acids in cattle milk/meat and milk productivity. This article describes the development of a real-time PCR assay for genotyping SNP rs41255693 in cattle SCD gene with allele-specific TaqMan probes. The use of this assay allows to significantly reduce the time needed for cattle genotyping compared to the commonly used PCR-RFLP method.
- Добавил в систему: Климов Евгений Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | agrfood-05-01-014.pdf | 358,5 КБ | 26 декабря 2019 [KlimovEA] |