Filamentous versus Spherical Morphology: A CaseStudy of the Recombinant A/WSN/33 (H1N1) Virus

Kordyukova, L.V.; Mintaev, R.R.; Rtishchev, A.A.; Kunda, M.S.; Ryzhova, N.N.; Abramchuk, S.S.; Serebryakova, M.V.; Khrustalev, V.V.; Khrustaleva, T.A.; Poboinev, V.V.; Markushin, S.G.; Voronina, O.L.

Авторы: Kordyukova Larisa V., Mintaev Ramil R., Rtishchev Artyom A., Kunda Marina S., Ryzhova Natalia N., Abramchuk Sergei S., Serebryakova Marina V., Khrustalev Vladislav V., Khrustaleva Tatyana A., Poboinev Victor V., Markushin Stanislav G., Voronina Olga L.
Журнал: Microscopy and Microanalysis
Том: 26
Номер: 2
Год издания: 2020
Издательство: Cambridge University Press
Местоположение издательства: United Kingdom
Первая страница: 297
Последняя страница: 309
DOI: 10.1017/S1431927620000069
Аннотация: Influenza A virus is a serious human pathogen that assembles enveloped virions on the plasma membrane of the host cell. The pleiomorphicmorphology of influenza A virus, represented by spherical, elongated, or filamentous particles, is important for the spread of the virus innature. Using fixative protocols for sample preparation and negative staining electron microscopy, we found that the recombinant A/WSN/33 (H1N1) (rWSN) virus, a strain considered to be strictly spherical, may produce filamentous particles when amplified in the allantoiccavity of chicken embryos. In contrast, the laboratory WSN strain and the rWSN virus amplified in Madin–Darby canine kidney cells exhibiteda spherical morphology. Next-generation sequencing (NGS) suggested a rare Ser126Cys substitution in the M1 protein of rWSN, whichwas confirmed by the mass spectrometric analysis. No structurally relevant substitutions were found by NGS in other proteins of rWSN.Bioinformatics algorithms predicted a neutral structural effect of the Ser126Cys mutation. The mrWSN_M1_126S virus generated after theintroduction of the reverse Cys126Ser substitution exhibited a similar host-dependent partially filamentous phenotype. We hypothesize thata shortage of some as-yet-undefined cellular components involved in virion budding and membrane scission may result in the appearanceof filamentous particles in the case of usually “nonfilamentous” virus strains.
Добавил в систему: Кордюкова Лариса Валентиновна

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Filamentous versus Spherical Morphology: A CaseStudy of the Recombinant A/WSN/33 (H1N1) Virusстатья

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