|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Induction of a Protein-Targeted Catalytic Response in Autoimmune Prone Mice: Antibody-Mediated Cleavage of HIV-1 Glycoprotein GP120†статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 февраля 2020 г.
- Авторы: Ponomarenko Natalia A., Vorobiev Ivan I., Alexandrova Elena S., Reshetnyak Andrew V., Telegin Georgy B., Khaidukov Sergey V., Avalle Bérangère, Karavanov Alexander, Morse Herbert C., Thomas Daniel, Friboulet Alain, Gabibov Alexander G.
- Журнал: Biochemistry
- Том: 45
- Номер: 1
- Год издания: 2006
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 324
- Последняя страница: 330
- DOI: 10.1021/bi050675k
- Аннотация: We have induced a polyclonal IgG that degrades the HIV-1 surface antigen, glycoprotein gp120, by taking advantage of the susceptibility of SJL mice to a peptide-induced autoimmune disorder, experimental autoimmune encephalomyelitis (EAE). Specific pathogen-free SJL mice were immunized with structural fragments of gp120, fused in-frame with encephalitogenic peptide MBP(85-101). It has resulted in a pronounced disease-associated immune response against antigens. A dramatic increase of gp120 degradation level by purified polyclonal IgG from immunized versus nonimmunized mice has been demonstrated by a newly developed fluorescence-based assay. This activity was inhibited by anti-mouse immunoglobulin antibodies as well as by Ser- and His-reactive covalent inhibitors. A dominant proteolysis site in recombinant gp120 incubated with purified polyclonal IgG from immunized mice was shown by SDS-PAGE. The SELDI-based mass spectrometry revealed that these antibodies exhibited significant specificity toward the Pro484-Leu485 peptide bond. The sequence surrounding this site is present in nearly half of the HIV-I variants. This novel strategy can be generalized for creating a catalytic vaccine against viral pathogens.
- Добавил в систему: Воробьев Иван Иванович