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Laser measurements of erythrocyte aggregation in patients suffering from arterial hypertensionтезисы доклада
- Авторы: Lugovtsov A.E., Dyachuk L.I., Ermolinskiy P.B., Maslyanitsina A.I., Priezzhev A.V.
- Сборник: Book of Abstracts of the International Conference “Advanced Laser Technologies”
- Тезисы
- Год издания: 2019
- Место издания: Прага
- Первая страница: 171
- Последняя страница: 172
- DOI: 10.24411/9999-011A-2019-00138
- Аннотация: Erythrocyte reversible aggregation is one the major phenomena that affect blood microcirculation. Alterations in aggregation properties lead to changing the blood viscosity and, as a consequence, to changes in blood flow through vessels and capillaries. This can lead to significant impairment of blood function, which increases a risk of occurrence of vascular concomitant diseases, and even the mortality especially in the case of cardiovascular pathologies. The erythrocytes aggregation and interaction properties are expected to be impaired in patients with arterial hypertension (AH). Thus, it is necessary to control these parameters during diagnostics and treatment of cardiovascular diseases. In this work, complex studies of the alterations of aggregation parameters of erythrocytes in whole blood samples and of pair interaction of single cells in patients sufferingfrom such a socially significant disease as AH were conducted by laser methods.The light scattering (laser aggregometry) technique allows for assessing the parameterscharacterizing the ability of the erythrocytes to reversibly aggregate in large ensembles of the cells. All measurements were performed using the commercially available Rheoscan system(Rheomeditech, Korea). This technique is convenient, fast and relatively simple for in vitromeasuring the aggregation properties of erythrocytes. It allows to register the kinetics of the spontaneous aggregation (time dependence of light intensity forward scattered from a sample of whole blood at rest) and shear-induced disaggregation (shear stress dependence of light intensity backscattered from a sample of whole blood under shear flow) of erythrocytes for obtaining the characteristic time of aggregates formation (aggregation rate), aggregation index as well as hydrodynamic strength of aggregates [1].Home-made double channeled optical tweezers (OT) were used for measuring the aggregationvelocity as well as the interaction forces on cellular level [2]. OT are formed by two single-mode Nd:YAG lasers and a water-immersion objective with high numerical aperture. OT allow for freely manipulating the individual cells with a tightly focused laser beam without direct mechanical contact.In vitro measurements were performed with EDTA-stabilized human blood samples drawnfrom patients with AH (88 people) and practically healthy volunteers – control (18 people). It was shown that in AH-patients, the aggregation speed of the erythrocytes and forces of the cells interaction are significantly increased relative to those in the control group while the ability of erythrocytes to aggregate in large ensembles is significantly enhanced relative to that in the control group. Basing on the obtained results one can conclude that laser aggregometry and optical trapping and manipulation are appropriate techniques for estimating the blood aggregation properties in whole blood samples and on the level of individual cells. The work was financially supported by the Russian Foundation for Basic Research grant No.17-02-01200.
- Добавил в систему: Масляницына Анастасия Игоревна