|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Astrocytes dystrophy in ageing brain parallels impaired synaptic plasticityстатья
- Авторы: Popov Alexander, Brazhe Alexey, Denisov Pavel, Sutyagina Oksana, Lazareva Natalia, Verkhratsky Alexei, Semyanov Alexey
- Журнал: bioRxiv (The preprint server for biology)
- Год издания: 2020
- DOI: 10.1101/2020.08.05.237420
- Аннотация: Little is known about age-dependent changes in structure and function of astrocytes and of the impact of these into the cognitive decline in the senescent brain. The prevalent view on age-dependent increase in reactive astrogliosis and astrocytic hypertrophy requires scrutiny and detailed analysis. Using two-photon microscopy in conjunction with 3D reconstruction, Sholl and volume fraction analysis we demonstrate a significant reduction in the number and the length of astrocytic processes, in astrocytic territorial domains and in astrocyte-to-astrocyte coupling in the aged brain. Probing physiology of astrocytes with patch-clamp and Ca2+ imaging revealed deficits in K+ and glutamate clearance, and spatiotemporal reorganization of Ca2+ events in old astrocytes. These changes paralleled impaired synaptic long-term potentiation (LTP) in hippocampal CA1 in old mice. Our findings may explain astroglial mechanisms of age-dependent decline in learning and memory.
- Добавил в систему: Браже Алексей Рудольфович