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Early and late MRI changes in rat brain after prolonged seizures and nonspatial memory impairmentтезисы доклада
- Авторы: Suleymanova E., Gulyaev M., Chepurnova N.
- Сборник: SiNAPSA Neuroscience Conference
- Тезисы
- Год издания: 2011
- Первая страница: 167
- Аннотация: Prolonged epileptic seizures are known to cause neuronal death and lead to brain damage. Lesions in various brain regions can result in memory and cognitive impairment. We studied early and late changes of brain structures after prolonged severe seizures on lithium-pilocarpine model of status epilepticus (SE) in rats. To induce SE, Wistar rats were treated with LiCl i.p., and pilocarpine i.p 24 hours after. Control animals received saline instead of pilocarpine. Seizures were observed and scored for 2 hours. MRI study of rat brain was performed 2, 7 and 30 days after SE. High-resolution T2 images and T2- maps were obtained, and total damaged area, hippocampal volume, and T2 coefficients in several brain structures were calculated. A week after the MRI study, animals were tested in an open field. To investigate the ability of the animals to habituate to the new environment, the test was performed three times with 24-hour intervals. After SE induced by pilocarpine, the increase of T2 signal was found in hippocampus and associated structures. The patterns of brain damage in rats after SE varied considerably. All rats after SE demonstrated high motor activity in an open field and did not habituate in the new environment that could be the evidence of long-term nonspatial memory deficit. Rats with large increase of T2 signal and considerable early changes found by MRI, tended to demonstrate higher activity in the open field in comparison with rats with less pronounced early MRI changes.
- Добавил в систему: Гуляев Михаил Владимирович