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A novel mechanism of eukaryotic translation initiation that is neither m7G-cap-, nor IRES-dependentстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 июля 2013 г.
- Авторы: Terenin IM, Andreev DE, Dmitriev SE, Shatsky IN
- Журнал: Nucleic Acids Research
- Том: 41
- Номер: 3
- Год издания: 2013
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 1807
- Последняя страница: 1816
- DOI: 10.1093/nar/gks1282
- Аннотация: Resistance of translation of some eukaryotic messenger RNAs (mRNAs) to inactivation of the cap-binding factor eIF4E under unfavorable conditions is well documented. To date, it is the mechanism of internal ribosome entry that is predominantly thought to underlay this stress tolerance. However, many cellular mRNAs that had been considered to contain internal ribosome entry sites (IRESs) failed to pass stringent control tests for internal initiation, thus raising the question of how they are translated under stress conditions. Here, we show that inserting an eIF4G-binding element from a virus IRES into 5'-UTRs of strongly cap-dependent mRNAs dramatically reduces their requirement for the 5'-terminal m(7)G-cap, though such cap-independent translation remains dependent on a vacant 5'-terminus of these mRNAs. Importantly, direct binding of eIF4G to the 5'-UTR of mRNA makes its translation resistant to eIF4F inactivation both in vitro and in vivo. These data may substantiate a new paradigm of translational control under stress to complement IRES-driven mechanism of translation.
- Добавил в систему: Шатский Иван Николаевич