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DNA damage and p21WAF1/CIP1/SDI1 in experimental injury of the rat adrenal cortex and trauma‐associated damage of the human adrenal cortexстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 февраля 2021 г.
- Авторы: Didenko Vladimir V., Wang Xiangdong, Yang Lianqing, Hornsby Peter J.
- Журнал: Journal of Pathology
- Том: 189
- Номер: 1
- Год издания: 1999
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 119
- Последняя страница: 126
- Аннотация: Abstract In vivo models are needed to study the reactions of tissues to DNA damage, such as the induction of the cyclin‐dependent kinase inhibitor p21, indicating potential repair of the damage, versus apoptosis, indicating the elimination of the damaged cells. Damage to DNA occurs in tissues during shock, sepsis, and other critical medical conditions. Previous studies have found evidence of damage to the cortex of adrenal glands from organ donors who had undergone severe trauma prior to death. The present experiment studied rats under experimental interventions of clinical relevance to patients with conditions that put them at risk for damage to the adrenal glands. These interventions comprised ischaemia and reperfusion injury, sepsis following caecal ligation and puncture, acute pancreatitis, and administration of chemical agents (zymosan and acrylonitrile). All the interventions caused an increase in p21 mRNA as assessed by northern blotting and in situ hybridization. Increased nuclear p21 protein was shown by immunohistochemistry. All the interventions caused damage to DNA, as shown by labelling of available 3′ termini of single‐strand breaks with terminal transferase. The number of cells undergoing apoptosis, visualized by ligation of a hairpin oligonucleotide probe to double‐strand breaks in DNA, was much lower. In rat adrenal glands, apoptotic cells were infrequent under all the conditions studied. They were more abundant in human organ donor adrenal glands that were previously shown to have extensive DNA damage accompanied by induction of p21. The similarity of the effects of a wide variety of surgical interventions and chemical agents suggest a common pathophysiological mechanism which is not specific to the initiating injury. Experimental injury of the rat adrenal cortex provides a model for investigating the role of organ DNA damage and of mediators of the response to DNA damage, such as p21.
- Добавил в систему: Didenko Vladimir Vladimirovich