Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysine

Galkina, S.I.; Fedorova, N.V.; Ksenofontov, A.L.; Serebryakova, M.V.; Golenkina, E.A.; Stadnichuk, V.I.; Baratova, L.A.; Sud’ina, G.F.

Авторы: Galkina Svetlana I., Fedorova Natalia V., Ksenofontov Alexander L., Serebryakova Marina V., Golenkina Ekaterina A., Stadnichuk Vladimir I., Baratova Ludmila A., Sud’ina Galina F.
Журнал: Cells
Том: 10
Год издания: 2021
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 563
DOI: 10.3390/cells10030563
Аннотация: During infection or certain metabolic disorders, neutrophils can escape from blood vessels,invade and attach to other tissues. The invasion and adhesion of neutrophils is accompanied andmaintained by their own secretion. We have previously found that adhesion of neutrophils tofibronectin dramatically and selectively stimulates the release of the free amino acid hydroxylysine.The role of hydroxylysine and lysyl hydroxylase in neutrophil adhesion has not been studied, norhave the processes that control them. Using amino acid analysis, mass spectrometry and electronmicroscopy, we found that the lysyl hydroxylase inhibitor minoxidil, the matrix metalloproteinaseinhibitor doxycycline, the PI3K/Akt pathway inhibitors wortmannin and the Akt1/2 inhibitor anddrugs that affect the actin cytoskeleton significantly and selectively block the release of hydroxylysineand partially or completely suppress spreading of neutrophils. The actin cytoskeleton effectors andthe Akt 1/2 inhibitor also increase the phenylalanine release. We hypothesize that hydroxylysinerelease upon adhesion is the result of the activation of lysyl hydroxylase in interaction with matrixmetalloproteinase, the PI3K/Akt pathway and intact actin cytoskeleton, which play important rolesin the recruitment of neutrophils into tissue through extracellular matrix remodeling.
Добавил в систему: Галкина Светлана Ивановна

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Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysineстатья Исследовательская статья

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