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A physicochemical, structural, microbiological and kinetic study of hen egg white lysozyme in complexes with alginate and chitosanстатья
- Авторы: Filatova Lyubov Y., Balabushevich Nadezhda G., Klyachko Natalia L.
- Журнал: Biocatalysis and Biotransformation
- Год издания: 2021
- Издательство: Taylor & Francis
- Местоположение издательства: United Kingdom
- DOI: 10.1080/10242422.2021.1909001
- Аннотация: Complexes of hen egg white lysozyme (HEWL) with alginate (ALG) and chitosan (CS) (naturalbiodegradable polysaccharides) are important as antimicrobial agents in oral, intranasal and buccaladministration. The HEWL–ALG, HEWL–ALG–Ca, [HEWL–ALG]–CS, and [HEWL–ALG–Ca]–CScomplexes have been investigated using a combination of physicochemical methods (fluorescentspectroscopy, circular dichroism, microscopy, turbidimetry, microbiology, in vitro cytotoxicityassay). The complexes obtained were non-toxic negatively charged microparticles with highHEWL entrapment efficiency. The [HEWL–ALG]–CS and [HEWL–ALG–Ca]–CS complexes werefound to have the altered tertiary structure of HEWL (and/or changes in the polarity of themicroenvironment of Trp residues in HEWL molecules). The HEWL entrapment in all studiedcomplexes was carried out with retention of the HEWL activity. Under the long–term storageconditions (1.5 years at 4 C), Caþþ cations and CS stabilized HEWL. Under physiological conditions,the [HEWL–ALG]–CS and [HEWL–ALG–Ca]–CS complexes demonstrated sustained releaseof HEWL and resistance to protease action. The [HEWL–ALG]–CS and [HEWL–ALG–Ca]–CS complexeswere highly active against Gram-negative Escherichia coli cells. The data obtained areimportant in the development of antibacterial drugs based on lytic enzymes
- Добавил в систему: Филатова Любовь Юрьевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Filatova_2021.pdf | Filatova_2021.pdf | 2,2 МБ | 27 мая 2021 [Balabushevich] |