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DNA Double-Strand Breaks Coupled with PARP1 and HNRNPA2B1 Binding Sites Flank Coordinately Expressed Domains in Human Chromosomesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Tchurikov Nickolai A., Kretova Olga V., Fedoseeva Daria M., Sosin Dmitri V., Grachev Sergei A., Serebryakova Marina V., Romanenko Svetlana A., Vorobieva Nadezhda V., Kravatsky Yuri V.
- Журнал: PLoS Genetics
- Том: 9
- Номер: 4
- Год издания: 2013
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: e1003429
- DOI: 10.1371/journal.pgen.1003429
- Аннотация: Genome instability plays a key role in multiple biological processes and diseases, including cancer. Genome-wide mapping of DNA double-strand breaks (DSBs) is important for understanding both chromosomal architecture and specific chromosomal regions at DSBs. We developed a method for precise genome-wide mapping of blunt-ended DSBs in human chromosomes, and observed non-random fragmentation and DSB hot spots. These hot spots are scattered along chromosomes and delimit protected 50-250 kb DNA domains. We found that about 30% of the domains (denoted forum domains) possess coordinately expressed genes and that PARP1 and HNRNPA2B1 specifically bind DNA sequences at the forum domain termini. Thus, our data suggest a novel type of gene regulation: a coordinated transcription or silencing of gene clusters delimited by DSB hot spots as well as PARP1 and HNRNPa2B1 binding sites.
- Добавил в систему: Серебрякова Марина Васильевна