Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing

Sheveleva, A.; Kudryavtseva, A.; Speranskaya, A.; Belenikin, M.; Melnikova, N.; Chirkov, S.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 мая 2014 г.

Авторы: Sheveleva A., Kudryavtseva A., Speranskaya A., Belenikin M., Melnikova N., Chirkov S.
Журнал: Virus Genes
Том: 47
Номер: 2
Год издания: 2013
Издательство: Springer Nature
Местоположение издательства: Switzerland
Первая страница: 385
Последняя страница: 388
DOI: 10.1007/s11262-013-0946-7
Аннотация: The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5′-terminal nucleotides. This region was amplified using 5′RACE kit and sequenced by the Sanger method. Genomic RNA released from immunocaptured PPV particles was employed for generation of cDNA library using TransPlex Whole transcriptome amplification kit (WTA2, Sigma-Aldrich). The entire Pk genome has identity level of 92.8–94.5 % when compared to the complete nucleotide sequences of other PPV-W isolates (W3174, LV-141pl, LV-145bt, and UKR 44189), confirming a high degree of variability within the PPV-W strain. The isolates Pk and LV-141pl are most closely related. The Pk has been found in a wild plum (Prunus domestica) in a new region of Russia indicating widespread dissemination of the PPV-W strain in the European part of the former USSR.
Добавил в систему: Сперанская Анна Сергеевна

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Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencingстатья

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