Multidrug-Resistant Klebsiella pneumoniae Causing Severe Infections in the Neuro-ICU

Astashkin, T.I.; Fursova, N.K.; Ershova, O.N.; Aleksandrova, I.A.; Savin, I.A.; Novikova, T.S.; Fedyukina, G.N.; Kislichkina, A.A.; Fursov, M.V.; Kuzina, E.S.; Biketov, S.F.; Dyatlov, I.A.

Авторы: Astashkin T.I., Fursova N.K., Ershova O.N., Aleksandrova I.A., Savin I.A., Novikova T.S., Fedyukina G.N., Kislichkina A.A., Fursov M.V., Kuzina E.S., Biketov S.F., Dyatlov I.A.
Журнал: Antibiotics
Том: 10
Номер: 8
Год издания: 2021
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Первая страница: 1
Последняя страница: 17
Номер статьи: 979
DOI: 10.3390/antibiotics10080979
Аннотация: The purpose of this study was the identification of genetic lineages and antimicrobial resistance (AMR) and virulence genes in Klebsiella pneumoniae isolates associated with severe infections in the neuro-ICU. Susceptibility to antimicrobials was determined using the Vitek-2 instrument. AMR and virulence genes, sequence types (STs), and capsular types were identified by PCR. Whole-genome sequencing was conducted on the Illumina MiSeq platform. It was shown that K. pneumoniae isolates of ST14K2, ST23K57, ST39K23, ST76K23, ST86K2, ST218K57, ST219KL125/114, ST268K20, and ST2674K47 caused severe systemic infections, including ST14K2, ST39K23, and ST268K20 that were associated with fatal incomes. Moreover, eight isolates of ST395K2 and ST307KL102/149/155 were associated with manifestations of vasculitis and microcirculation disorders. Another 12 K. pneumoniae isolates of ST395K2,KL39, ST307KL102/149/155, and ST147K14/64 were collected from patients without severe systemic infections. Major isolates (n = 38) were XDR and MDR. Beta-lactamase genes were identified: blaSHV (n = 41), blaCTX-M (n = 28), blaTEM (n = 21), blaOXA-48 (n = 21), blaNDM (n = 1), and blaKPC (n = 1). The prevalent virulence genes were wabG (n = 41), fimH (n = 41), allS (n = 41), and uge (n = 34), and rarer, detected only in the genomes of the isolates causing severe systemic infections—rmpA (n = 8), kfu (n = 6), iroN (n = 5), and iroD (n = 5) indicating high potential of the isolates for hypervirulence.
Добавил в систему: Ершова Ольга Николаевна

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