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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 Tatстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или ScopusДата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.
- Авторы: Kurnaeva M.A., Zalevsky A.O., Arifulin E.A., Lisitsyna O.M., Tvorogova A.V., Shubina M.Y., Bourenkov G.P., Tikhomirova M.A., Potashnikova D.M., Kachalova A.I., Musinova Y.R., Golovin A.V., Vassetzky Y.S., Sheval E.V.
- Журнал: Journal of Virology
- Том: 96
- Номер: 1
- Год издания: 2022
- Издательство: American Society for Microbiology
- Местоположение издательства: United States
- Номер статьи: e0150521
- DOI: 10.1128/jvi.01505-21
- Аннотация: During evolution, viruses had to adapt to an increasingly complex environment of eukaryotic cells. Viral proteins that need to enter the cell nucleus or associate with nucleoli possess nuclear localization signals (NLSs) and nucleolar localization signals (NoLSs) for nuclear and nucleolar accumulation, respectively. As viral proteins are relatively small, acquisition of novel sequences seems to be a more complicated task for viruses than for eukaryotes. Here, we carried out a comprehensive analysis of the basic domain (BD) of HIV-1 Tat to show how viral proteins might evolve with NLSs and NoLSs without an increase in protein size. The HIV-1 Tat BD is involved in several functions, the most important being the transactivation of viral transcription. The BD also functions as an NLS, although it is substantially longer than a typical NLS. It seems that different regions in the BD could function as NLSs due to its enrichment with positively charged amino acids. Additionally, the high positive net charge inevitably causes the BD to function as an NoLS through a charge-specific mechanism. The integration of NLSs and NoLSs into functional domains enriched with positively charged amino acids might be a mechanism that allows the condensation of different functional sequences in small protein regions and, as a result, to reduce protein size, influencing the origin and evolution of NLSs and NoLSs in viruses.
- Добавил в систему: Шеваль Евгений Валерьевич