Branched-chain amino acids administration suppresses endurance exercise-related activation of ubiquitin proteasome signaling in trained human skeletal muscle

Lysenko, E.; Vepkhvadze, T.; Lednev, E.; Vinogradova, O.; Popov, D.

Авторы: Lysenko EA, Vepkhvadze TF, Lednev EM, Vinogradova OL, Popov DV
Журнал: J Physiol Sci
Том: 68
Номер: 1
Год издания: 2018
Первая страница: 43
Последняя страница: 53
DOI: 10.1007/s12576-016-0506-8
Аннотация: We tested whether postexercise ingestion of branched-chain amino acids (BCAA <10 g) is sufficient to activate signaling associated with muscle protein synthesis and suppress exercise-induced activation of mechanisms associated with proteolysis in endurance-trained human skeletal muscle. Nine endurance-trained athletes performed a cycling bout with and without BCAA ingestion (0.1 g/kg). Postexercise АССSer79/222 phosphorylation (endogenous marker of AMPK activity) was increased (3-fold, P<0.05) in both sessions. No changes were observed in IGF1 mRNA isoform expression or phosphorylation of the key anabolic markers—p70S6K1Thr389 and eEF2Thr56—between the sessions. BCAA administration suppressed exercise-induced expression of mTORC1 inhibitor DDIT4 mRNA, eliminated activation of the ubiquitin proteasome system, detected in the control session as decreased FOXO1Ser256 phosphorylation (0.83-fold change, P<0.05) and increased TRIM63 (MURF1) expression (2.4-fold, P<0.05). Therefore, in endurance-trained human skeletal muscle, postexercise BCAA ingestion partially suppresses exercise-induced expression of PGC-1a mRNA, activation of ubiquitin proteasome signaling, and suppresses DDIT4 mRNA expression.
Добавил в систему: Лысенко Евгений Алексеевич

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Branched-chain amino acids administration suppresses endurance exercise-related activation of ubiquitin proteasome signaling in trained human skeletal muscleстатья

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