|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Effect of lithium chloride on the volume of ischemic stroke in ratsтезисы доклада
- Авторы: Cherpakov R.A., Grebenchikov O.A., Ershov A.V., Shabanov A.K., Antonova V.V., Kuzovlev A.N.
- Сборник: 40th International Symposium on Intensive Care & Emergency Medicine 2021
- Серия: S1
- Том: 25
- Тезисы
- Год издания: 2021
- Место издания: Springer Science and Business Media LLC
- Первая страница: 41
- Последняя страница: 42
- Номер статьи: P104
- DOI: 10.1186/s13054-021-03769-1
- Аннотация: Introduction: The aim of the study was to evaluate the effect of lith- ium chloride in various dosages on the volume of brain damage in ischemic stroke in rats.Methods: The study used male rats weighing 315 ± 13.5 g. The long focal ischemia model was used. The animals were split into five groups: false-operated (median incision in the projection of the carotid artery without ischemia), control (model of ischemic stroke with the intro- duction of 0.9% NaCl) and three groups with the introduction of lith- ium chloride at concentrations of 4.2 mg/kg, 21 mg/kg and 63 mg/kg, respectively. Lithium chloride was administered immediately after the termination of the occlusion of the middle cerebral artery and then every 24 h until euthanasia. To assess the degree of brain damage, the animals underwent magnetic resonance imaging (MRI) on day 2, and brain sections stained with 2,3,5-triphenyltetrazolium chloride were evaluated on day 7 after euthanasia. Results: According to MRI data, lithium chloride at a dose of 4.2 mg/ kg did not significantly affect the volume of ischemic stroke and peri- focal edema in relation to the control group on day 2 (p = 0.9). When using a dose of 21 mg/kg, the volume of stroke (p = 0.04) and perifo- cal edema was significantly lower (p = 0.03) than in the control group (by 25% and 18%, respectively). Lithium chloride at a dose of 63 mg/kg significantly reduced the volume of stroke (45%, p = 0.004) and peri- focal edema (35%, p = 0.007). When determining the volume of the lesion on day 7, the data were comparable with the results obtained on day 2. When using a dose of 21 mg/kg, the stroke volume was 20% lower (p = 0.04) than in the control group. Lithium chloride at a dose of 63 mg/kg reduced stroke volume by 40% (p = 0.0037). Conclusions: Lithium chloride at dosages of 21 mg/kg and 63 mg/ kg significantly reduced the volume of ischemic stroke and perifocal edema of the brain, but when using a concentration of 63 mg/kg, the effect was more pronounced.
- Добавил в систему: Черпаков Ростислав Александрович