Is the mitochondrial membrane potential (∆ψ) correctly assessed? Intracellular and intramitochondrial modifications of the ∆Ψ probe, rhodamine 123

Zorova, L.D.; Demchenko, E.A.; Korshunova, G.A.; Tashlitsky, V.N.; Zorov, S.D.; Andrianova, N.V.; Popkov, V.A.; Babenko, V.A.; Pevzner, I.B.; Silachev, D.N.; Plotnikov, E.Y.; Zorov, D.B.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.

Авторы: Zorova Ljubava D., Demchenko Evgenia A., Korshunova Galina A., Tashlitsky Vadim N., Zorov Savva D., Andrianova Nadezda V., Popkov Vasily A., Babenko Valentina A., Pevzner Irina B., Silachev Denis N., Plotnikov Egor Y., Zorov Dmitry B.
Журнал: International Journal of Molecular Sciences
Том: 23
Номер: 1
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 482
DOI: 10.3390/ijms23010482
Аннотация: The mitochondrial membrane potential (∆Ψ) is the driving force providing the electrical component of the total transmembrane potential of hydrogen ions generated by proton pumps which is utilized by the ATP synthase. The role of ∆Ψ is not limited to its role in bioenergetics, since it takes part in other important intracellular processes, which leads to the mandatory requirement of homeostasis of ∆Ψ. Conventionally, ∆Ψ in living cells is estimated by the fluorescence of probes such as rho-damine 123, tetramethylrodamine, etc. However, when assessing the fluorescence, the possibility of intracellular/intramitochondrial modification of the rhodamine molecule is not taken into account. Such changes were revealed in this work, in which a comparison of normal (astrocytic) and tumor (glioma) cells was carried out. Fluorescent microscopy, flow cytometry, and mass spectrometry revealed significant modifications of rhodamine molecules developing over time, which were prevented by amiodarone, apparently due to blocking the release of xenobiotics from the cell and their transformation with the participation of cytochrome P450. Obviously, an important role in these processes is played by increased retention of rhodamines in tumor cells. Our data require careful evaluation of mitochondrial ∆Ψ potential based on the assessment of the fluorescence of the mitochondrial probe.
Добавил в систему: Зоров Дмитрий Бориcович

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Is the mitochondrial membrane potential (∆ψ) correctly assessed? Intracellular and intramitochondrial modifications of the ∆Ψ probe, rhodamine 123статья

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