Аннотация:resent paper is dedicated to the numerical study of transient free-molecular gas flow through the microchannel with a series of barriers, oscillating perpendicular to the axis of the channel. Phases of barriers motion are arranged in a specific way to obtain a “wave-like” motion of barriers system. Time-dependent flow rate pattern is studied for different values of problem parameters — oscillation frequencies, number of barriers, distance between them, pressure drop in the channel. It is obtained that pattern of gas flow rate significantly depends on the ratio of characteristic “wavespeed” of barriers system motion and the thermal speed of molecules. It is shown that frequency of output pulsatile flow may be significantly higher than the frequency of barriers mechanical oscillations, depending on the number of barriers, which can be used in practice for creation of high-frequency pulsatile flows. Similarly to authors previous paper Kosyanchuk, et al. (2017) it was obtained that motion of barriers system can lead to separation of gas mixtures with different molecular masses. In contrast to “quasi-stationary” (time-periodic) flow regime (at times around 100-1000 oscillation periods), it is shown that in transient regime (at times less than 10-100 oscillation periods) separation factor can be significantly higher. Moreover, separation effect in transient regime is observed even for the values of defining parameters, which do not grant separation in quasi-stationary flow regime.