Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucoseстатья
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Аннотация:O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 andstudied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharidewas established:where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-Dglucose (Bacillosamine) in which N-2 is acetylatedand N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veroniiSHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in theavailable databases and found to be in agreement with the OPS structure.