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Content of Free Fetal DNA in Maternal Blood and Expression of DNA Recognition Receptors ZBP-1 in Placental Tissue in Preeclampsia and Preterm Laborстатья
- Авторы: Baev O.R., Karapetian A.O., NIZIEVA N.V., Sadecova Krasniy A.A.>.A.M
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 168
- Год издания: 2019
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- DOI: 10.1007/s10517-019-04665-z
- Аннотация: We evaluated the content of cell-free fetal DNA in maternal blood and expression of ZBP-1receptors in the placental tissue of women with uncomplicated pregnancy, preeclampsia, andpreterm labor. The study included 16 women with preeclampsia (early and late-onset preeclampsia, 8 cases each), 16 women with preterm labor, and 21 women with uncomplicatedpregnancy. The concentration of cell-free fetal DNA was measured by PCR by detectinghypermethylated region of the RASSF1A gene. Immunohistochemistry was performed onparaffin-embedded sections of the placenta samples using primary polyclonal antibodies toZBP-1. Significant increase in the level of cell-free fetal DNA was found in women withpreeclampsia (both early and late-onset form) in comparison with uncomplicated pregnancy.The concentration of cell-free fetal DNA in preterm labor group did not differ from thecontrol group; however, it was significantly lower than in early-onset preeclampsia, but notlate preeclampsia. Immunohistochemical study showed higher expression of ZBP-1 in thevillus syncytiotrophoblast in early-onset preeclampsia in comparison with that in pretermlabor group (p=0.006). Fragments of damaged placental cells, predominantly trophoblast,enter maternal circulation and are the source of cell-free fetal DNA and a potential ligand forZBP-1, which leads to further cell damage and the formation of a vicious circle. The increasein the content of cell-free fetal DNA in maternal blood and ZBP-1 expression in the syncytiotrophoblast in preeclampsia are interrelated processes reflecting impaired morphofunctionalstate of the placenta.
- Добавил в систему: Баев Олег Радомирович