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Voltage generation by photosystem I complexes immobilized onto a millipore filter under continuous illuminationстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Zaspa Andrey, Vitukhnovskaya Liya, Mamedova Aida, Allakhverdiev Suleyman I., Semenov Alexey, Mamedov Mahir
- Журнал: International Journal of Hydrogen Energy
- Том: 47
- Номер: 22
- Год издания: 2022
- Издательство: Pergamon Press Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 11528
- Последняя страница: 11538
- DOI: 10.1016/j.ijhydene.2022.01.175
- Аннотация: Here we measured the long-term photovoltage (DV) generation by photosynthetic pigment-protein complex of photosystem I (PS I) from cyanobacterium Synechocystis sp. PCC 6803directly. Prior to measurements PS I was immobilized onto a Millipore membrane filter (MF)and sandwiched between two semiconductor indium tin oxide (ITO) electrodes. The abilityof ITO|PS I-MF|ITO system to generate a stable DV over a 50 min period of irradiation bywhite light was detected in the presence of cytochrome c6, methyl viologen and disaccharide trehalose. Re-injection of a fresh buffer into ITO|PS I-MF|ITO preserves the immobilized PS I functionality after six months of storage at 23 C in the dark. Thedeveloped system represents a simple bio-hybrid solar converter to obtain voltage signals under long-term, periodic illumination. This approach can also be used to couple photoactive (light-driven enzymes) and catalytic modules to generate H2 under the steady-state conditions.
- Добавил в систему: Семенов Алексей Юрьевич