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Development of lateral flow immunoassay for rapid control and quantification of the presence of the colorant Sudan I in spices and seafoodстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 февраля 2017 г.
- Авторы: Berlina Anna N., Zherdev Anatoly V., Chuanlai Xu, Eremin Sergey A., Dzantiev Boris B.
- Журнал: Food Control
- Том: 73
- Год издания: 2017
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 247
- Последняя страница: 253
- DOI: 10.1016/j.foodcont.2016.08.011
- Аннотация: The lateral flow immunoassay for colorant Sudan I detection was developed using the monoclonal antibody to Sudan I conjugated with gold nanoparticles. Conditions of the assay were optimized for both qualitative control and quantification of Sudan I in food matrixes with minimal dilution of the extracting media (final content of methanol – 50%). In the case of photometric registration, the limit of Sudan I detection in extracts was 2.5 ng/mL, and the working range of determined concentrations was 13–240 ng/mL. The average error of measurements varied from 5.5 to 16.9%. The visual limit of detection, which is the minimum concentration causing absence of visible coloration at the test zone, was 1 ?g/mL. The assay efficiency was confirmed by its application to determine Sudan I content in highly colored spices (turmeric, curry) and seafood (caviar, mussels, fish); the reached limit of detection was 10 ng/g. Thus, the developed assay is suitable as a rapid, inexpensive tool for revealing Sudan I quantification by mobile laboratories and in out-of-laboratory conditions.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | FoodCont73B_247.pdf | FoodCont73B_247.pdf | 819,3 КБ | 28 января 2017 [Sergei_A_EREMIN] |