Microgels in tandem with enzymes: tuning adsorption of a pH- and thermoresponsive microgel for improved design of enzymatic biosensorsстатья
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Дата последнего поиска статьи во внешних источниках: 18 мая 2022 г.
Аннотация:We highlight benefits of microgel’s stimuli-responsiveness for surface modification and further engineering of microgel-enzyme biosensor constructs. Accordingly, the adsorption behavior of the pH- and temperature-sensitive poly(N-isopropylacrylamide-co-N-(3-dimethylaminopropyl)methacrylamide) microgel was examined by atomic force microscopy (AFM) and quartz-crystal microbalance with dissipation monitoring (QCM-D). An enhanced adsorption of the microgel onto relatively hydrophobic surfaces was observed at elevated pH and temperature as manifested by a high surface coverage (AFM) and a large adsorbed mass (QCM-D). This is a consequence of pronounced hydrophobization of the microgel as revealed by dynamic light scattering (DLS) and turbidimetry (cloud points). The subsequent electrostatic loading of the surface-bound microgel with enzymes was examined by means of QCM-D and demonstrated highly-capacious integration of biomolecules into the microgel films. Finally, the biosensor responses of the microgel-enzyme films fabricated onto screen-printed graphite electrodes were assessed to demonstrate a biorelated application. Thus, a comprehensive conceptual understanding was attained on the key points: (1) how the stimuli-responsive properties of the microgel correlate with its amount deposited onto the surface, (2) what determines the highly-capacious loading of the surface-bound microgel with the enzymes, and ultimately (3) how the tandem of the stimuli-sensitive microgels and enzymes can be used for easy engineering of biosensor systems.