Аннотация:Variable fluorescence of chlorophyll (CF) of the photosynthetic apparatus is an ample source of valuable information onphysiological condition of photosynthetic organisms. Currently, the most widespread CF-based technique is represented byrecording pulse-amplitude modulated (PAM) induction of CF by saturating light. The CF-based monitoring techniques areincreasingly employed for characterization of performance and stress resilience of microalgae in microalgal biotechnology.Analysis of CF induction curves reveals the fate of light energy absorbed by photosynthetic apparatus, the proportions ofthe energy that have been utilized for photochemistry (culture growth), and heat dissipated by photoprotective mechanisms.Hence CF and its derived parameters are an accurate proxy of the metabolic activity of the photosynthetic cell and the engagementof photoprotective mechanisms. This information is a solid foundation for making decisions on the microalgal culturemanagement during the lab-scale and industrial-scale cultivation. Applications of CF and PAM include the monitoring ofstressor (high light, nutrient deprivation, extreme temperatures, etc.) effects for assessment of the culture robustness. It alsoserves as a non-invasive express test for gauging the effect of assorted toxicants in microalgae. This approach is becomingwidespread in ecological toxicology and environmental biotechnology, particularly for bioprospecting strains capable ofthe destruction of dangerous pollutants such as pharmaceuticals. In the review, we discuss the advantages and drawbacksof using CF-based methods for assessment of the culture conditions. Special attention is paid to the potential caveats andapplicability of different variations of CF and PAM measurements for solving problems of microalgal biotechnology