Tissue homeostasis in sponges: Quantitative analysis of cell proliferation and apoptosis

Melnikov, N.P.; Bolshakov, F.V.; Frolova, V.S.; Skorentseva, K.V.; Ereskovsky, A.V.; Saidova, A.A.; Lavrov, A.I.

Авторы: Melnikov Nikolai P., Bolshakov Fyodor V., Frolova Veronika S., Skorentseva Kseniia V., Ereskovsky Alexander V., Saidova Alina A., Lavrov Andrey I.
Журнал: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Год издания: 2022
Издательство: John Wiley & Sons Ltd.
Местоположение издательства: United Kingdom
DOI: 10.1002/jez.b.23138
Аннотация: Tissues of multicellular animals are maintained due to a tight balance between cell proliferation and programmed cell death. Sponges are early branching metazoans essential to understanding the key mechanisms of tissue homeostasis. This paper is dedicated to the comparative analysis of proliferation and apoptosis in intact tissues of two sponges, Halisarca dujardinii (class Demospongiae) and Leucosolenia variabilis (class Calcarea). Labeled nucleotides EdU and anti-phosphorylated histone 3 antibodies reveal a considerable number of cycling cells in intact tissues of both species. Quantitative DNA staining reveals the classic cell cycle distribution curve. The main type of cycling cells are choanocytes - flagellated cells of the aquiferous system. The rate of proliferation remains constant throughout various areas of sponge bodies that contain choanocytes. The EdU tracking experiments conducted in H. dujardinii indicate that choanocytes may give rise to mesohyl cells through migration. The number of apoptotic cells in tissues of both species is insignificant, although being comparable to the renewing tissues of other animals. In vivo studies with TMRE and CellEvent Caspase-3/7 indicate that apoptosis might be independent of mitochondrial outer membrane permeabilization. Altogether, a combination of confocal laser scanning microscopy and flow cytometry provides a quantitative description of cell proliferation and apoptosis in sponges displaying either rapid growth or cell turnover.
Добавил в систему: Лавров Андрей Игоревич

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