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Feedback between fluidity of membranes and transcription of the desB gene for the ω3-desaturase in the cyanobacterium Synechocystisстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Mironov K.S., Maksimov E.G., Maksimov G.V., Los D.A.
- Журнал: Molecular Biology
- Том: 46
- Номер: 1
- Год издания: 2012
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 134
- Последняя страница: 141
- DOI: 10.1134/S002689331201013X
- Аннотация: Cells of prokaryotes (including cyanobacteria) respond to a decrease in the environmental temperature by activation of multiple genes with a low-temperature response. A decrease in temperature causes a reduction of the cell membrane fluidity, which is maintained at an optimum level due to the activity of fatty acid (FA) desaturases. We studied a temperature-dependent expression of the desB gene for the ω3-desaturase in the cyanobacterium Synechocystis, which is able to synthesize polyunsaturated FA, as well as in its double mutant (desA −/desD −), defective in the Δ12- and Δ6-desaturase genes, for which the presence of only monounsaturated FA is typical. During a decrease in temperature, in wild type cells the amount of desB mRNA increased, reaching a maximum value at 24°C. In the desA −/desD − double mutant, an accumulation of the desB transcript was characterized by a maximum at 28–30°C. Thus, using the desB gene encoding the ω3-FA-desaturase, it was demonstrated that a temperature-dependent expression of genes responsible for the maintenance of an optimal fluidity of cell membranes is determined by a physical state of these membranes and is regulated by a feedback mode.
- Добавил в систему: Максимов Евгений Георгиевич