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Fabrication of chitosan-hyaluronic acid nanoparticles andencapsulation into nanoparticles of dinitrosyl iron complexes aspotential cardiological drugsстатья
- Авторы: Akentieva Natalia P., Gizatullin Аrthur R., Sanina Natalia A., Dremova Nadezhda N., Torbov VladimirI, Shkondina Natalia I., Zhelev Nikolai, Aldoshin Sergei M.
- Журнал: Journal of Nanomedicine & Nanotechnology
- Том: 7
- Номер: 3
- Год издания: 2020
- Первая страница: 199
- Последняя страница: 210
- DOI: 10.22038/nmj.2020.07
- Аннотация: Objective(s): Currently, the development of nanoparticles for the stabilization and targeted delivery of cardiacdrugs has gained significance. The present study aimed to develop nontoxic nanoparticles based on chitosan-hyaluronic acid (HA), encapsulate dinitrosyl iron complexes (DNICs, donors NO) into the nanoparticlesto increase the stability and effectiveness of their action, and assess the effect of the nanoparticle-DNICcomplex on the cell viability of cardiomyocytes.Materials and Methods: Nanoparticles were obtained from chitosan-HA using the ionotropic gelationtechnology, and the morphology and size of the nanoparticles were determined using electron microscopy.The DNICs were built into the nanoparticles using the physical association method, and the stability of thenanoparticle-DNIC complexes and NO release was investigated using the electrochemical method.Results: Analysis by the electron microscopy showed that the nanoparticles were homogeneous in terms ofshape and had an optimal size of ~100 nanometers. In addition, the incorporation of the DNICs into thecomposition of the nanoparticles significantly increased the stability of the DNICs, while also prolonging thegeneration of NO and enhancing the yield of nitrogen monoxide. Fluorescence analysis indicated that thechitosan-HA nanoparticles increased the cell viability of rat cardiomyocytes.Conclusion: The nanoparticles were fabricated from chitosan and HA. The encapsulation of the DNICs intothe composition of the nanoparticles could stabilize these compounds, while prolonging and increasingthe generated nitric oxide. The nanoparticle-DNICs were water-soluble, biocompatible, biodegradable, and nontoxic, which could be used as potential cardiac drugs for the treatment of cardiovascular diseases
- Добавил в систему: Шкондина Наталья Ивановна