Novel mouse monoclonal antibodies specifically recognizing $\upbeta$-(1$\rightarrow$3)-D-glucan antigen

Matveev, A.L.; Krylov, V.B.; Khlusevich, Y.A.; Baykov, I.K.; Yashunsky, D.V.; Emelyanova, L.A.; Tsvetkov, Y.E.; Karelin, A.A.; Bardashova, A.V.; Wong Sarah, S.W.; Aimanianda, V.; Latgé, J.; Tikunova, N.V.; Nifantiev, N.E.

Авторы: Matveev Andrey L., Krylov Vadim B., Khlusevich Yana A., Baykov Ivan K., Yashunsky Dmitry V., Emelyanova Ljudmila A., Tsvetkov Yury E., Karelin Alexander A., Bardashova Alevtina V., Wong Sarah S.W., Aimanianda Vishukumar, Latgé Jean-Paul, Tikunova Nina V., Nifantiev Nikolay E.
Журнал: PLoS ONE
Том: 14
Номер: 4
Год издания: 2019
Издательство: Public Library of Science
Местоположение издательства: United States
Первая страница: e0215535
DOI: 10.1371/journal.pone.0215535
Аннотация: β-(1→3)-D-Glucan is an essential component of the fungal cell wall. Mouse monoclonal antibodies (mAbs) against synthetic nona-β-(1→3)-D-glucoside conjugated with bovine serum albumin (BSA) were generated using hybridoma technology. The affinity constants of two selected mAbs, 3G11 and 5H5, measured by a surface plasmon resonance biosensor assay using biotinylated nona-β-(1→3)-D-glucan as the ligand, were approximately 11 nM and 1.9 nM, respectively. The glycoarray, which included a series of synthetic oligosaccharide derivatives representing β-glucans with different lengths of oligo-β-(1→3)-D-glucoside chains, demonstrated that linear tri-, penta- and nonaglucoside, as well as a β-(1→6)-branched octasaccharide, were recognized by mAb 5H5. By contrast, only linear oligo-β-(1→3)-D-glucoside chains that were not shorter than pentaglucosides (but not the branched octaglucoside) were ligands for mAb 3G11. Immunolabelling indicated that 3G11 and 5H5 interact with both yeasts and filamentous fungi, including species from Aspergillus, Candida, Penicillium genera and Saccharomyces cerevisiae, but not bacteria. Both mAbs could inhibit the germination of Aspergillus fumigatus conidia during the initial hours and demonstrated synergy with the antifungal fluconazole in killing C. albicans in vitro. In addition, mAbs 3G11 and 5H5 demonstrated protective activity in in vivo experiments, suggesting that these β-glucan-specific mAbs could be useful in combinatorial antifungal therapy.
Добавил в систему: Лопухов Антон Владимирович

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Novel mouse monoclonal antibodies specifically recognizing $\upbeta$-(1$\rightarrow$3)-D-glucan antigenстатья