Raman microscopy shows that nitrogen-rich cellular inclusions in microalgae are microcrystalline guanine

Moudříková, Š.; Nedbal, L.; Solovchenko, A.; Mojzeš, P.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 мая 2017 г.

Авторы: Moudříková Šárka, Nedbal Ladislav, Solovchenko Alexei, Mojzeš Peter
Журнал: Algal Research
Том: 23
Год издания: 2017
Первая страница: 216
Последняя страница: 222
DOI: 10.1016/j.algal.2017.02.009
Аннотация: Microalgal cells possess a vast diversity of subcellular structures and cytoplasmic inclusions differing in theirmorphology, functionality, and composition, some of them giving rise to distinct Raman spectral signatures allowing their identification, localization, and visualization in situ. Here, we show that certain Raman features that we observed in Raman spectra of microalgae can be unambiguously attributed to guanine microcrystals because they are clearly distinct even from Raman fingerprints of other, closely related purine species. Using confocal Raman microscopy, we have localized crystalline guanine as a part of cellular inclusions in the chlorophyte Desmodesmus quadricauda and in the eustigmatophyte Trachydiscus minutus. We propose that this finding also explains the chemical nature of similar nitrogen-rich crystalline structures recently documented in a number of other chlorophyte species by energy-dispersive X-ray spectroscopy. To the best of our knowledge, this is the first Raman microscopy-based direct evidence of the presence of guanine microcrystalline inclusions within microalgal cells. We tentatively propose that the crystalline guanine serves as a very compact, long-term depot of nitrogen in microalgae. Simplicity of specimen preparation requiring no fixation, labeling, or staining of the cells predetermines Raman microscopy as a method of choice for more advanced studies of physiological role of guanine particles as well as other crystalline inclusions in situ within intact cells.
Добавил в систему: Соловченко Алексей Евгеньевич

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ЦЭМИ РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ЦЭМИ РАН

Raman microscopy shows that nitrogen-rich cellular inclusions in microalgae are microcrystalline guanineстатья

Прикрепленные файлы