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ВЛИЯНИЕ ОСВЕЩЕННОСТИ И АЗОТНОГО ГОЛОДАНИЯ НА РОСТ И НАКОПЛЕНИЕ АРАХИДОНОВОЙ КИСЛОТЫ У ОДНОКЛЕТОЧНОЙ ВОДОРОСЛИ PARIETOCHLORIS INCISAстатья
Статья опубликована в журнале из списка RSCI Web of Science
Статья опубликована в журнале из перечня ВАК- Авторы: Соловченко А.Е., Мерзляк М.Н., Чивкунова О.Б., Решетникова И.В., Хозина-Голдберг И., Диди-Коэн Ш., Коэн Ц.
- Журнал: Вестник Московского университета. Серия 16: Биология
- Номер: 1
- Год издания: 2008
- Издательство: Изд-во Моск. ун-та
- Местоположение издательства: М.
- Первая страница: 49
- Последняя страница: 53
- Аннотация: Parietochloris incisa is a unicellular, fresh water green alga, capable of accumulating high amounts of the valuable very long chain polyunsaturated arachidonic acid (AA) in triacylglycerols (TAG) of cytoplasmic oil-bodies. To find out the cultivation conditions providing maximum AA yield, the effects of light irradiance and N- availability on the dry weight (DW), chlorophyll, carotenoid and AA content have been studied. Under nitrogen starvation, TAG accounted for over 30% of dry weight (DW) and AA content became as high as ~ 55% of total fatty acids. From the standpoint of biomass accumulation, light intensity of ca. 400 µE m-2 s-1 was found to be optimal for growing P. incisa on complete medium. Lower light intensities (or higher cell density of inoculum) resulted in higher AA yield when the alga was cultivated on nitrogen-free media. In the absence of nitrogen, algal cells were unable to cope with high light and suffered from photooxidative damage, whereas the nitrogen-sufficient culture survived under such illumination conditions probably due to accumulation of carotenoids. Nitrogen-deprived P. incisa cells displayed elevated sensitivity to light.
- Добавил в систему: Соловченко Алексей Евгеньевич